');--grs:50px;--gtaos:105px;--gtaosi:-105px;--gtaof:210px;--gbes:calc(100% + 105px);--gbef:calc(100% + 210px);--gbet:translateX(-105px);--gbeti:translateX(105px);--vgc:repeat(12, 1fr);--gc1:repeat(12, 1fr);--gc2:repeat(6, 1fr);--gc3:repeat(4, 1fr);--gc3h:1/4;--gc4:repeat(3, 1fr);--gcm:8.333%;--gac:1fr;--gcw50:50%;--gc2tp:0 calc(var(--grs) * 2) 0 0;--gc2sw:var(--gbes);--gc2slpl:0 0 0 105px;--gc2srpf:0 105px 0 0;--gc2sof:1;--gc2sol:2;--gc50:4/span 6;--gc48a:1/5;--gc48b:5/-1;--gc84a:1/9;--gc84b:9/-1;--gc39a:1/4;--gc39b:4/-1;--gc93a:1/10;--gc93b:10/-1;--gc615a:1/7;--gc615b:8/-1;--gc516a:1/6;--gc516b:7/-1;--tppr:0;--bgp:42px;--s_cn:0;--s_ces:4px;--s_cest:4px 4px 0 0;--s_cesb:0 0 4px 4px;--s_cs:8px;--s_cm:12px;--s_cl:16px;--s_cls:16px 0 0 16px;--s_cle:0 16px 16px 0;--s_clt:16px 16px 0 0;--s_cel:28px;--s_celt:28px 28px 0 0;--s_cf:50%;--m_ds1:50ms;--m_ds2:100ms;--m_ds3:150ms;--m_ds4:200ms;--m_dm1:250ms;--m_dm2:300ms;--m_dm3:350ms;--m_dm4:400ms;--m_dl1:450ms;--m_dl2:500ms;--m_dl3:550ms;--m_dl4:600ms;--m_de1:700ms;--m_de2:800ms;--m_de3:900ms;--m_de4:1000ms;--m_el:0,0,1,1;--m_es:0.2,0,0,1;--m_esa:0.3,0,1,1;--m_esd:0,0,0,1;--m_ee:0.2,0,0,1;--m_eea:0.3,0,0.8,0.15;--m_eed:0.05,0.7,0.1,1;--m_elg:0.4,0,0.2,1;--m_elga:0.4,0,1,1;--m_elgd:0,0,0.2,1;--cc_w:255,255,255;--cc_b:0,0,0;--c_p:1,31,48;--c_op:255,255,255;--c_pc:203,230,253;--c_opc:1,31,48;--c_s:67,81,200;--c_os:255,255,255;--c_sc:223,224,255;--c_osc:0,9,101;--c_t:83,97,98;--c_ot:255,255,255;--c_tc:215,229,230;--c_otc:17,30,31;--c_e:179,38,30;--c_oe:255,255,255;--c_ec:249,222,220;--c_oec:65,14,11;--c_co:109,123,124;--c_b:255,255,255;--c_ob:17,30,32;--c_su:249,249,249;--c_osu:17,30,32;--c_suv:215,229,230;--c_osuv:60,73,74;--c_is:38,51,53;--c_iso:239,241,242;--c_ip:175,202,224;--c_sh:0,0,0;--c_st:1,31,48;--c_cov:187,201,202;--c_scr:0,0,0;--c_suchs:225,234,236;--c_such:231,239,241;--c_suc:245,245,245;--c_sucl:249,249,249;--c_scls:255,255,255;--c_sub:249,249,249;--c_sud:209,220,222}:root ::-webkit-scrollbar,:root ::-webkit-scrollbar-corner{background:0 0;width:9pt;height:9pt}body,frameset{padding:0;margin:0;color:rgb(var(--c_ob));background:rgb(var(--c_b));font-family:var(--t_bmf);font-size:var(--t_bms);line-height:var(--t_bmlh);font-weight:var(--t_bmw);letter-spacing:var(--t_bmt)}body{--space_nav_top:0px;--space_nav_bottom:0px;--space_nav_left:0px;--space_nav_right:0px;--C_X:0px;--CXV:visible;overflow-x:hidden;overflow-y:var(--CXV)}footer,header{display:flex;justify-content:flex-start}score-pointer{z-index:11000}addium-header .main *,addium-menu-footer p,addium-menu-footer p *,score-text *{-webkit-user-select:text!important;-moz-user-select:text!important;user-select:text!important}:root{--t_dlf:var(--t_tb);--t_dllh:68px;--t_dls:63px;--t_dlt:-0.5px;--t_dlw:400;--t_dmf:var(--t_tb);--t_dmlh:42px;--t_dms:35px;--t_dmt:0.25px;--t_dmw:400;--t_hlf:var(--t_tp);--t_hllh:42px;--t_hls:35px;--t_hlt:0.25px;--t_hlw:400;--t_hmf:var(--t_tp);--t_hmlh:27px;--t_hms:21px;--t_hmt:0.25px;--t_hmw:400;--t_tlf:var(--t_tm);--t_tllh:24px;--t_tls:16px;--t_tlt:0.15px;--t_tlw:500;--t_tmf:var(--t_tm);--t_tmlh:normal;--t_tms:13px;--t_tmt:0.45px;--t_tmw:500;--t_llf:var(--t_tp);--t_lllh:16px;--t_lls:12px;--t_llt:0.5px;--t_llw:500;--t_lmf:var(--t_tp);--t_lmlh:16px;--t_lms:11px;--t_lmt:0.5px;--t_lmw:500;--t_blf:var(--t_tp);--t_bllh:30px;--t_bls:21px;--t_blt:0.1px;--t_blw:400;--t_bmf:var(--t_tp);--t_bmlh:24px;--t_bms:17px;--t_bmt:0.1px;--t_bmw:400;--grs:50px;--gtaos:105px;--gtaosi:-105px;--gtaof:210px;--gbes:calc(100% + 105px);--gbef:calc(100% + 210px);--gbet:translateX(-105px);--gbeti:translateX(105px);--vgc:repeat(4, 1fr);--gc1:1fr;--gc2:1fr;--gc3:1fr;--gc3h:1/-1;--gc4:1fr 1fr;--gc5:1fr;--gcm:8.333%;--gac:auto;--gcw50:100%;--gc2tp:0;--gc2sw:100%;--gc2slpl:calc(var(--grs) / 2) 0;--gc2srpf:calc(var(--grs) / 2) 0;--gc2sof:2;--gc2sol:1;--gc50:1/-1;--gc48a:1/-1;--gc48b:1/-1;--gc84a:1/-1;--gc84b:1/-1;--gc39a:1/-1;--gc39b:1/-1;--gc93a:1/-1;--gc93b:1/-1;--gc615a:1/-1;--gc615b:1/-1;--gc516a:1/-1;--gc516b:1/-1;--bgp:18px}main{width:100%}addium-header score-form[type="0"] score-input-container.col-1,addium-header score-form[type="0"] score-input-container.col-2,addium-header score-form[type="0"] score-input-container.col-3,addium-header score-form[type="0"] score-input-container.col-4,addium-header score-form[type="0"] score-input-container.col-5,addium-header score-form[type="1"] score-input-container.col-1,addium-header score-form[type="1"] score-input-container.col-2,addium-header score-form[type="1"] score-input-container.col-3,addium-header score-form[type="1"] score-input-container.col-4,addium-header score-form[type="1"] score-input-container.col-5,addium-header score-form[type="2"] score-input-container.col-1,addium-header score-form[type="2"] score-input-container.col-2,addium-header score-form[type="2"] score-input-container.col-3,addium-header score-form[type="2"] score-input-container.col-4,addium-header score-form[type="2"] score-input-container.col-5,addium-header score-form[type="3"] score-input-container.col-1,addium-header score-form[type="3"] score-input-container.col-2,addium-header score-form[type="3"] score-input-container.col-3,addium-header score-form[type="3"] score-input-container.col-4,addium-header score-form[type="3"] score-input-container.col-5,score-banner-element section p,score-dialog-element fieldset,score-dialog-element section p,score-input-container.col-1 score-input,score-input-container.col-2 score-input,score-input-container.col-3 score-input,score-input-container.col-4 score-input,score-input-container.col-5 score-input,score-input.DJA input,score-input.DJB input,score-input.DJC input,score-input.DJE input,score-input.DJH input,score-input.DJI input,score-install section p,score-loader section p,score-push-notification section p,score-sidesheet-drawer section p{margin:0}addium-header score-form label,score-input.hidden,score-select.hidden,score-textarea.hidden{display:none!important}addium-header[type="0"] .graphics3d figure,addium-header[type="0"] .score_media_element figure,addium-header[type="0"] figure figure,addium-header[type="1"] .graphics3d figure,addium-header[type="1"] .score_media_element figure,addium-header[type="1"] figure figure,score-form[type="6"],score-form[type="7"]{padding:0}addium-header{--hm:-42px;--hp:0;--hw:calc(100% + 84px);--hmm:60px;--hmw:calc(100% - 120px);--h1mw:725px;view-transition-name:header;display:grid;grid-template-columns:var(--vgc);grid-column:1/-1;width:100%}addium-header score-form{width:auto;max-width:450px;padding:28px 0!important}addium-header score-form score-input input{--cw:0!important}addium-header score-form[type="0"] .DI_,addium-header score-form[type="1"] .DI_,addium-header score-form[type="2"] .DI_,addium-header score-form[type="3"] .DI_,score-text td p,score-text th p{margin:0!important}addium-header score-form[type="6"],addium-header score-form[type="7"]{max-width:350px}addium-header[type="0"] .graphics3d,addium-header[type="0"] .score_media_element,addium-header[type="0"] figure,addium-header[type="1"] .graphics3d,addium-header[type="1"] .score_media_element,addium-header[type="1"] figure,addium-header[type="2"] .graphics3d,addium-header[type="2"] .score_media_element,addium-header[type="2"] figure,addium-header[type="3"] .graphics3d,addium-header[type="3"] .score_media_element,addium-header[type="3"] figure,score-teaser.GX .score_media_element{z-index:0}addium-header[type="0"] .main,addium-header[type="1"] .main,addium-header[type="2"] .main,addium-header[type="3"] .main{z-index:1;flex-direction:column}addium-header[type="0"] .main h1,addium-header[type="1"] .main h1,addium-header[type="2"] .main h1,addium-header[type="3"] .main h1{font-family:var(--t_dmf);font-size:var(--t_dms);line-height:var(--t_dmlh);font-weight:var(--t_dmw);letter-spacing:var(--t_dmt);max-width:var(--h1mw)}addium-header[type="0"],addium-header[type="1"]{grid-template-rows:repeat(6,1fr);margin:var(--hm) 0 0 var(--hm);padding:var(--hp);width:var(--hw)}addium-header[type="0"] .graphics3d,addium-header[type="0"] .score_media_element,addium-header[type="0"] figure,addium-header[type="1"] .graphics3d,addium-header[type="1"] .score_media_element,addium-header[type="1"] figure{grid-area:1/1/-1/-1;margin:0;width:100%}addium-header[type="0"] .main,addium-header[type="1"] .main{margin:0 var(--hmm);width:var(--hmw);display:block;grid-area:3/1/-1/-1}addium-header[type="0"] .main h1,addium-header[type="0"] .main h3,addium-header[type="1"].inverted .main h1,addium-header[type="1"].inverted .main h3{color:rgb(var(--cc_w))}addium-header[type="0"] .main h3.tts::before,addium-header[type="1"].inverted .main h3.tts::before{background:rgb(var(--cc_w))}addium-header[type="2"] .main,addium-header[type="3"] .main{display:block;grid-row:1/span 2}addium-header[type="2"] .main{margin:3pc 18px;width:calc(100% - 36px);grid-column:1/-1}addium-header[type="2"] .graphics3d,addium-header[type="2"] .score_media_element,addium-header[type="2"] figure{margin:6px;width:calc(100% - 12px);display:block;grid-column:1/-1;grid-row-end:span 6;padding:0!important}addium-header[type="2"]+score-video-external{margin:-9pt;width:calc(100% + 24px);grid-column:1/-1}addium-header[type="3"] .main{grid-column:1/7;height:min-content;align-self:center;margin:0 18px}addium-header[type="3"] .graphics3d,addium-header[type="3"] .score_media_element,addium-header[type="3"] figure{grid-column:7/-1}:root{--sv:visible;--tti:translate(0, 0);--ttc:translate(-50%, -50%);--ttcx:translate(-50%, 0);--ttcy:translate(0, -50%);--ttvx:translateX(-50vw);--ttl:translate(-100%, 0);--ttr:translate(100%, 0);--ttt:translate(0, -100%);--ttb:translate(0, 100%);--tra:rotate(45deg);--trb:rotate(-45deg);--trc:rotate(90deg);--trd:rotate(-90deg);--tsa:scaleX(0);--tsb:scaleX(1);--tsc:scaleY(0);--tsd:scaleY(1);--bxa:0 2px 4px -1px rgba(0, 0, 0, 0.2),0 1px 10px 0 rgba(0, 0, 0, 0.12),0 4px 5px 0 rgba(0, 0, 0, 0.14);--ba:1px solid var(--cb);--bb:1px solid var(--cw);--bc:1px solid var(--cg1);--bd:1px solid var(--cg2);--be:1px solid var(--cg4);--c_st0:0;--c_st1:0.05;--c_st2:0.08;--c_st3:0.11;--c_st4:0.12;--c_st5:0.15;--c_bs0:drop-shadow(0px 0px 0px rgba(0, 0, 0, 0.2)) drop-shadow(0px 0px 0px rgba(0, 0, 0, 0.14)) drop-shadow(0px 0px 0px rgba(0, 0, 0, 0.12));--c_bs1:drop-shadow(0px 2px 1px rgba(0, 0, 0, 0.2)) drop-shadow(0px 1px 1px rgba(0, 0, 0, 0.14)) drop-shadow(0px 1px 3px rgba(0, 0, 0, 0.12));--c_bs2:drop-shadow(0px 3px 3px rgba(0, 0, 0, 0.2)) drop-shadow(0px 3px 4px rgba(0, 0, 0, 0.14)) drop-shadow(0px 1px 8px rgba(0, 0, 0, 0.12));--c_bs3:drop-shadow(0px 3px 5px rgba(0, 0, 0, 0.2)) drop-shadow(0px 6px 10px rgba(0, 0, 0, 0.14)) drop-shadow(0px 1px 18px rgba(0, 0, 0, 0.12));--c_bs4:drop-shadow(0px 5px 5px rgba(0, 0, 0, 0.2)) drop-shadow(0px 8px 10px rgba(0, 0, 0, 0.14)) drop-shadow(0px 3px 14px rgba(0, 0, 0, 0.12));--c_bs5:drop-shadow(0px 7px 8px rgba(0, 0, 0, 0.2)) drop-shadow(0px 12px 17px rgba(0, 0, 0, 0.14)) drop-shadow(0px 5px 22px rgba(0, 0, 0, 0.12));--s_dec1:1;--s_dec2:1;--s_dc1:0.12;--s_dc2:0.38;--s_de:0;--s_ho:0.08;--s_fo:0.12;--s_po:0.12;--s_do:0.16;--s_scr:0.5;--g_l0:linear-gradient(to top, rgba(0, 0, 0, 0.5) 0, rgba(0, 0, 0, 0.15) 100%);--g_l1:linear-gradient(to top, rgba(0, 0, 0, 0.5) 0, rgba(0, 0, 0, 0) 50%);--g_l2:linear-gradient(to top, rgba(0, 0, 0, 0.66) 0, rgba(0, 0, 0, 0.33) 50%);--t_tb:AddiumSerif;--t_tp:AddiumSans;--t_tm:AddiumMono;--t_dlf:var(--t_tb);--t_dllh:136px;--t_dls:126px;--t_dlt:-1px;--t_dlw:400;--t_dmf:var(--t_tb);--t_dmlh:68px;--t_dms:63px;--t_dmt:-0.5px;--t_dmw:400;--t_dsf:var(--t_tb);--t_dslh:42px;--t_dss:35px;--t_dst:0.25px;--t_dsw:400;--t_hlf:var(--t_tp);--t_hllh:68px;--t_hls:63px;--t_hlt:-0.5px;--t_hlw:400;--t_hmf:var(--t_tp);--t_hmlh:42px;--t_hms:35px;--t_hmt:0.25px;--t_hmw:400;--t_hsf:var(--t_tp);--t_hslh:27px;--t_hss:21px;--t_hst:0.25px;--t_hsw:400;--t_tlf:var(--t_tm);--t_tllh:28px;--t_tls:22px;--t_tlt:0;--t_tlw:400;--t_tmf:var(--t_tm);--t_tmlh:24px;--t_tms:16px;--t_tmt:0.15px;--t_tmw:500;--t_tsf:var(--t_tm);--t_tslh:normal;--t_tss:13px;--t_tst:0.45px;--t_tsw:500;--t_llf:var(--t_tp);--t_lllh:20px;--t_lls:14px;--t_llt:0.1px;--t_llw:500;--t_lmf:var(--t_tp);--t_lmlh:16px;--t_lms:12px;--t_lmt:0.5px;--t_lmw:500;--t_lsf:var(--t_tp);--t_lslh:16px;--t_lss:11px;--t_lst:0.5px;--t_lsw:500;--t_blf:var(--t_tp);--t_bllh:34px;--t_bls:24px;--t_blt:0;--t_blw:400;--t_bmf:var(--t_tp);--t_bmlh:30px;--t_bms:21px;--t_bmt:0.1px;--t_bmw:400;--t_bsf:var(--t_tp);--t_bslh:24px;--t_bss:17px;--t_bst:0.1px;--t_bsw:400;--i_s1:svg-url('');--grs:50px;--gtaos:105px;--gtaosi:-105px;--gtaof:210px;--gbes:calc(100% + 105px);--gbef:calc(100% + 210px);--gbet:translateX(-105px);--gbeti:translateX(105px);--vgc:repeat(12, 1fr);--gc1:repeat(12, 1fr);--gc2:repeat(6, 1fr);--gc3:repeat(4, 1fr);--gc3h:1/4;--gc4:repeat(3, 1fr);--gcm:8.333%;--gac:1fr;--gcw50:50%;--gc2tp:0 calc(var(--grs) * 2) 0 0;--gc2sw:var(--gbes);--gc2slpl:0 0 0 105px;--gc2srpf:0 105px 0 0;--gc2sof:1;--gc2sol:2;--gc50:4/span 6;--gc48a:1/5;--gc48b:5/-1;--gc84a:1/9;--gc84b:9/-1;--gc39a:1/4;--gc39b:4/-1;--gc93a:1/10;--gc93b:10/-1;--gc615a:1/7;--gc615b:8/-1;--gc516a:1/6;--gc516b:7/-1;--tppr:0;--bgp:42px;--s_cn:0;--s_ces:4px;--s_cest:4px 4px 0 0;--s_cesb:0 0 4px 4px;--s_cs:8px;--s_cm:12px;--s_cl:16px;--s_cls:16px 0 0 16px;--s_cle:0 16px 16px 0;--s_clt:16px 16px 0 0;--s_cel:28px;--s_celt:28px 28px 0 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This webinar operates under the assumption that all participants have a working knowledge of water activity and moisture absorption. Therefore, the webinar content will establish basic principles of the effects of water activity on salmonella water activity. To learn the basics of water activity and how it’s used, please refer to Water Activity 101: Mastering the Basics.
While salmonella cannot grow or proliferate in an environment held below a certain water activity level, it can survive and remain present. One particularly tricky aspect of food production is that many products are subsequently used as ingredients in other food products. If one ingredient or inclusion contains bacteria, the final product will too.
The higher the thermal resistance of a bacteria, the harder it is to inactivate that bacteria within a substance. Therefore, we performed a study of thermal resistance using two different types of substances: all-purpose flour and peanut butter. We created isotherms to map the relationship between the water activity and moisture content in these two samples.
In this experiment, we examined the impact of temperature on the capacitance of polymer film, relative humidity, and water activity. The thermal resistance of the flour and the peanut butter differed, even though they were subject to the same temperature increase. This reinforces that in food production, it is important to remember that the thermal resistance of bacteria will differ depending on the substance.
Inactivation of pathogens is achieved through a complicated process: inoculation, equilibration (e.g., control humidity), fabrication (e.g., grind, mill, etc.), re-equilibration (five to twelve days), and treatment through bench scale (iso- and non-iso-) and pilot-scale.
When working with any substance, inoculation is a crucial step and affects the resistance of any bacterial organisms. Several product treatments are available to inoculate and further test bacterial thermal resistance. These treatments include bench scale, isothermal, and pilot scale. All of these involve monitoring the temperature and humidity in an environment. A product’s starting water activity level will directly impact the thermal resistance of the present bacteria.
In an example using almonds, roasting the nuts resulted in a low moisture system, low water activity, a high temperature, and a low bacterial population. In further experiments, it was determined that higher humidity resulted in double the kill rate of the bacteria.
The relationship between water activity, moisture content, and humidity is complex and requires precise measurements and full attention.
The webinar presents five theoretical alternatives to validate a process: (1) pathogen challenge in actual plant; (2) surrogate challenge in actual plant; (3) pathogen challenge in pilot-scale; (4) surrogate challenge in pilot-scale; and (5) time-temperature measurement and inactivation model.
The first option is a non-starter because we never want to introduce an actual pathogen into our facilities, so the presenter focuses on the latter three options. The three key takeaways from this webinar are (1) Water affects salmonella thermal resistance through water activity, moisture content, and process humidity; (2) the relationships between water activity, moisture content, and process humidity are complex; and (3) other factors (e.g., composition, structure) also affect resistance.